If you look at the back of the cassette you will see soft spots that are the valves. The cycler behind the membrane can exert pressure on these soft spots thus effectively shutting down the flow from different tubes. It has a multi stage pumping system that draws fluid, moves it to another chamber where is accumulated and then squeezed out into the drain tube. My neighbor was involved in designing and building the sterile enclosures that robotically build the cassettes.
Thanks for this and informative replies to my other postings. I thought it might be something like this, but the only spots that obviously looked flexible were the large ones. Is there a way to get specs that show which spot controls which flow? I guess it wouldn't be too hard to reverse engineer, but I haven't taken a close look.